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. 2001 Nov;39(11):3982–3986. doi: 10.1128/JCM.39.11.3982-3986.2001

FIG. 1.

FIG. 1

Western immunoblot analysis of representative serum samples. (A) dually E. chaffeensis and HGE agent IFA-positive sera 3 and 4 (Table 1); (B) E. chaffeensis IFA-positive, HGE agent IFA-negative sera 14 and 16; (C) E. chaffeensis IFA-negative, HGE agent IFA-positive sera 17 and 19. DH82 cells, HL60 cells, and pET29-transformed E. coli were negative controls. Ech, purified E. chaffeensis; HGE, purified HGE agent; rP30, affinity-purified recombinant fusion protein of E. canis; rP44, affinity-purified recombinant fusion protein of the HGE agent. Antigens (15 μg of DH82 cells, E. coli, purified E. chaffeensis, and the HGE agent and 0.3 μg of rP30 and rP44) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Numbers on the left are molecular masses (in kilodaltons) based on broad-range prestained standards (Bio-Rad Laboratories, Richmond, Calif.).