TABLE 1.
Sample | IFA titer
|
Western immunoblotting
|
PCR (culture) result
|
State of residence or presumed tick exposure | |||||
---|---|---|---|---|---|---|---|---|---|
E. chaffeensis | HGE agent | Approximate molecular size(s) (kDa) of reacting antigen(s)
|
rP30 | rP44 | E. chaffeensis | HGE agent | |||
Purified E. chaffeensis | Purified HGE agent | ||||||||
1 | 5,120 | 320 | 40, 46, 55, 58, 110 | 42 | +a | − | NAb | NA | NA |
2 | 2,560 | 1,280 | 28, 47 | 30, 45, 72 | +a | − | NA | NA | NA |
3 | 5,120 | 2,560 | 26, 28, 34, 42, 52 | 47, 49, 55 | +a | − | NA | NA | NA |
4 | 1,280 | 640 | 30, 44, 47, 110 | 49, 55, 72 | +a | − | NA | NA | NA |
5 | 1,280 | 640 | 44, 55 | 45, 47, 72 | +a | − | NA | NA | NA |
6 | 2,560 | 1,280 | 27, 28, 55, 74 | 55 | +a | − | NA | NA | NA |
7 | 5,120 | 320 | 23, 26, 30, 45, 55, 58, 90 | +a | − | NA | NA | NA | |
8 | 5,120 | 320 | 30, 40, 45, 55 | 72 | +a | − | NA | NA | NA |
9 | 1,280 | 640 | 45, 48, 58, 110 | 42, 47, 55 | +a | − | +c | NA | NA |
10 | 640 | 320 | 28, 45, 55 | +a | − | +c | NA | NA | |
11 | 640 | 1,280 | 45, 58 | 49, 55 | + | − | NA | NA | Arkansas |
12 | 2,560 | 320 | 28, 40, 44 | + | − | NA | NA | Missouri | |
13 | 5,120 | 320 | 44 58, 74, 90, 110 | + | − | NA | NA | Missouri | |
14 | 640 | 28, 32, 45, 58, 110 | + | − | NA | NA | Texas | ||
15 | 160 | 30, 40, 58 | + | − | +d | NA | Washington, D.C. | ||
16 | 160 | 40, 55, 110 | + | − | +(+)d | NA | Florida | ||
17 | 160 | 47 | − | + | NA | −e | New York | ||
18 | 320 | 49 | − | + | NA | +e | New York | ||
19 | 160 | 44, 47, 49 | − | + | NA | − (+)e | New York |
Data from reference (20).
NA, not available.
Performed at MRL Reference Laboratory.
Performed at CDC.
Performed at New York Medical College.