Table 2.
The orthogonal experiment design table (L9 (33) for carbon
source and nitrogen source concentration and results analysis
| Level | Bran (g/L) | Peptone (g/L) | Corn steep powder (g/L) | C. butyricum viable counts (× 107 cfu/mL) | C. butyricum spores (× 107 cfu/mL) |
|---|---|---|---|---|---|
| 1 | 20 | 15 | 5 | 5.8 | 4.4 |
| 2 | 10 | 10 | 15 | 8.5 | 7.4 |
| 3 | 20 | 5 | 15 | 6.1 | 5.2 |
| 4 | 10 | 15 | 10 | 7.4 | 5.7 |
| 5 | 15 | 15 | 15 | 8.2 | 6.1 |
| 6 | 20 | 10 | 10 | 5.6 | 3.9 |
| 7 | 15 | 10 | 5 | 6.5 | 3.5 |
| 8 | 15 | 5 | 10 | 3.1 | 2.7 |
| 9 | 10 | 5 | 5 | 5.8 | 3.5 |
| ‾K1a | 7.23 | 5.00 | 6.03 | ||
| ‾K2a | 5.93 | 6.87 | 5.37 | ||
| ‾K3a | 5.83 | 7.13 | 7.60 | ||
| RA | 1.40 | 2.13 | 2.23 | ||
| ‾K1b | 5.53 | 3.80 | 3.80 | ||
| ‾K2b | 4.10 | 4..93 | 4.10 | ||
| ‾K3b | 4.50 | 5.40 | 6.23 | ||
| RB | 1.43 | 1.60 | 2.43 |
K value stands for the mean value, for example, K1a, K2a and K3a correspond to A are the mean values of factor A, at level 1, level 2, and level 3, respectively, and so on. R stand for range value, correspond to the different between the highest k value and the lowest k value. English letter A and B in rang analysis results stand for the results of C. butyricum DL-1 viable counts and spore yields, respectively