Fig. 1. Accelerated atherosclerosis in M-Jak2-deficient mice.

a, b Representative images of freshly isolated aortic arches isolated from M-Jak2 KO and WT control mice fed 3 weeks of HCD starting at 6 weeks of age. Scale bar, 5 mm. c, d Representative images of en face Oil-red-O staining of atherosclerotic plaques in the lesser curvature of the aortic arch after 3 weeks of HCD. Scale bar, 1 mm. e, f Representative images of en face Oil-red-O staining of atherosclerotic plaque area in the descending aorta after 16 weeks of HCD. Scale bar, 1 mm. g Quantification of atherosclerotic plaque area (mm²) in the aortic arch after 3 weeks of HCD (n = 18–19/genotype). h Quantification of atherosclerotic plaque area in the descending aorta after 16 weeks of HCD, expressed as a percentage of total area (n = 18–19/genotype). Statistical analysis: two-tailed unpaired t tests were performed. Data are presented as the mean ± SEM, ****P < 0.0001. See also Fig. 2, Supplementary Figs. S1, S2, S3, and S4.