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Specificity of recombinant NcSRS2 against anti-N. caninum serum. ELISA using NLA or the recombinant NcSRS2 expressed by Ba/SRS2p44 or Ba/SRS2p41 was carried out using anti-N. caninum (A) or anti-T. gondii (B) mouse serum (1:250). The antigens (Ag) from the culture supernatants (sup) and the cell lysates (cell) of recombinant baculovirus-infected Sf9 cells were prepared as described in Materials and Methods.
