FIG. 3.

Examination of bovine sera. Minimum and maximum values (Min-Max), 25 and 75% percentiles (25%–75%), and median values of ELISA using the NLA or the recombinant NcSRS2 prepared from Ba/SRS2p44-infected cells (SRS2) are shown. Bovine sera that were N. caninum IFAT positive (P) (n = 40) and negative (N) (n = 40), which were randomly selected, were examined by ELISA. The cutoff values were determined by the mean value plus two standard deviations from bovine sera that were IFAT negative (recombinant NcSRS2 ELISA, optical density = 0.189; NLA ELISA, optical density = 0.371).