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. 2022 Feb 2;13:807330. doi: 10.3389/fphar.2022.807330

FIGURE 4.

FIGURE 4

Selective deletion of vHPC A2ARs triggers anxiety-like behaviors. (A,B) Behavior of the four groups of mice in the open-field test. Caffeine induced anxiogenic behavior with decreased time in the center (B) and distance in the center (A) only in EGFP mice. (C,D) Behavior of the four groups of mice in the Elevated Plus Maze (EPM) test. Caffeine decreased the percentage of open-arm entries (C) and the percentage of time spent in the open arms (D) only in EGFP mice. (E) Behavior of the four groups of mice in the Novel Object Recognition (NOR) test. The time spent exploring a novel object was increased by caffeine in EGFP and CRE mice during the testing trial. (F) Behavior of the four groups of mice in the Y-maze test. The time spent in the novel arm was increased by caffeine in EGFP and CRE mice during the retrieval phase. (G) Expression of AAV-CRE or AAV-EGFP in the vHPC (scale bar = 500 μm). Data are presented as the mean ± SEM; n = 7 mice per group; *p < .05, two-way ANOVA, Bonferroni post hoc t-test.