FIGURE 11.

Electro-acupuncture (EA) treatment activates the MS/VDB-DG regulating Cho/Cr in 5 × FAD mice. (A,D,G,J) ¹H MRS exhibits NAA peak at 2.02 ppm, Cho peak at 3.2 ppm, and Cr peak at 3.05 ppm in MS/VDB and DG of groups pre- and post-treatment. The ratio of NAA/Cr in MS/VDB (B,C) and Cho/Cr in DG (E,F) were significantly decreased pre-treatment. The ratios of NAA/Cr and Cho/Cr in MS/VDB (H,I) and DG (K,L) were significantly increased in the 5 × FAD + EA group. With inhibition of the MS/VDB-DG cholinergic neural circuit, the ratios of Cho/Cr in MS/VDB and DG were decreased. Data are represented as mean ± SD, n = 10/group, and each circle represents a mouse (*p < 0.05 and ^**p < 0.01 vs WT group; ▲p < 0.05 and ▲▲p < 0.01 vs 5 × FAD group; ^#p < 0.05 and ^##p < 0.01 vs 5 × FAD + EA group; ^&P < 0.05 and ^&&p < 0.01 vs 5 × FAD + EA + hM4Di group).