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. 2022 Feb 2;13:770948. doi: 10.3389/fnagi.2021.770948

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Copyright © 2022 Li, Li, Dai, Yang, Liang, Wang, Liu, Chen and Tao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Electro-acupuncture (EA) treatment activates the MS/VDB-DG cholinergic neural circuit regulating M1 receptors in 5 × FAD mice. Representative images of M1 receptors (A) and M2 receptors (B) in the DG. Groups were subjected to immunofluorescence labeling with Dapi (Blue), M1 or M2 (red) staining. Scale bar = 100 μm. Quantified M1 positive cells (C) and M2 positive cells (D). Data are represented as mean ± SD, n = 4/group (^**p < 0.01 vs WT group; ▲p < 0.05 vs 5 × FAD group; ^#p < 0.05 vs 5 × FAD + EA group; ^&&p < 0.01 vs 5 × FAD + EA + hM4Di group).