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CRISPR-Cas9 gene disruption of CXCR4 in Jurkat T cells
(A) Jurkat cells were transduced with CXCR4 CRISPR or control vector and analyzed for CXCR4 surface expression by flow cytometry. (B) Surveyor nuclease assay detects indels in CXCR4 CRISPR-modified Jurkat cells. (C) Sanger sequencing and ICE analysis of CRISPR-Cas9-induced genome disruption by CXCR4. (D) HIV-1NL4-3 replication in PBMCs treated with CXCR4 CRISPR, as measured by p24 in supernatant.
