Fig. 5.

STAT3 activity is critical for expression of Hck in Shh MB, whereas STAT3‐driven Gli1 expression requires Smo activation. (A) Bar graphs of relative mRNA levels by RT‐PCR showing IL‐6 or constitutively activated STAT3 significantly promoted expression of Hck in Daoy cells (top and middle panels); NSC74859 treatment significantly decreased expression of Hck (bottom panel). Data are presented as mean ± SD; t‐tests were used to determine the significance. Results shown are representative of at least three independent experiments. (B) Sant‐1 treatment inhibited expression of Gli1 in Daoy cells with control vector or S3c. Results shown are representative of at least three independent experiments. (C) Schema depicting the interdependent regulation of STAT3 and Shh signaling.