TABLE 1.

Primers used in PCR reactions for flaA sequencing and expression of the respective protein

Target DNA	Primer (5′-3′)a	Location (bp)
Sequencing
1	ATA TAA GGA GTT GGT TTA CAT	−19–2
2	ATG AAA AGG AAA GCT AAA AGT	1–21
3	GCT ATT CTC AAT CAT CTG C	386–404
4	TTT ATG AGA CTA GCG GAA CT	860–879
5	TTA AAT AAA CCT TGC CAT CAA	1,438–1,418
6	TTT TCT AAA TCT AAT ATT TCC AT	1,117–1,094
7	CAT TTT ACT TGA AGC AAG AG	717–698
Expression
FlaABbia	CCG GAT CCG ATG GAT TAG CAG AGG GTT	67–85
	CCG GTA CCC TAA TTT TTC GGA GAT GAT TC	1,026–1,006
FlaABaA91	CCG GAT CCG ATG GAT TAA CAG AGG GC	67–84
	CCG GTA CCC TAA TTT TCT GAA GAT GAT TC	1,026–1,006
FlaABg46	CCG GAT CCG ATG GAT TGC CAG AAG GC	67–84
	CCG GTA CCC TAC TTT TTT AAG GAT GAT TC	1,029–1,009

^aUnderlining indicates BamHI and KpnI cleavage sites.