Figure 5:

Statistics of second pore capture. (a,b) The voltage bias (top) and the number of DNA nucleotides in the delivery channels and flow chamber (bottom) versus simulation time for end-threaded capture (panel a) and for folded capture (panel b). The time between captures is denoted by t_cap and the number of nucleotides in the flow chamber at the time of second capture, N_out. The images illustrate the DNA conformation 4 μs after second capture, when V₁ is changed to 0.6 V. These times are denoted with a dashed line. (c) Scatter plot of N_out versus t_cap for all 100 simulations. The simulations marked with green and blue arrows are the ones shown in panel a and b, respectively. The histograms show the distribution of the t_cap (top) and N_out (right) values. The cumulative probability of double nanopore capture reaches 100%, i.e., all 100 molecules were simultaneously captured in both nanopores.