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. 2022 Jan;10(2):90. doi: 10.21037/atm-21-6868

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2022 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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The identification and efficiency of FNDC5 gene transduction. (A) Immunofluorescence staining confirmed the successful overexpression of FNDC5 in BMSCs, and BMSCs-OE-FNDC5 had persistent stability in in vitro cultures. (B) qRT-PCR results exhibited the increased messenger RNA level of FNDC5 in BMSCs transfected by the constructed lentivirus. (C) Western blot images and semi-quantification analysis further confirmed the overexpression protein of FNDC5. **, P<0.01. Scale bar =100 µm. BMSCs-OE-FNDC5, FNDC5-overexpressing BMSCs.