FIG. 1.

Agarose gel electrophoresis patterns showing single PCR and MPCR amplification products for the S. aureus genes femB, ileS-2, and mecA amplified from a highly methicillin- and highly mupirocin-resistant S. aureus isolate. Lanes 1 to 3, PCR amplicons from femB, ileS-2, and mecA, respectively; lane 4, triplex PCR amplicons, i.e., femB, ileS-2, and mecA simultaneously amplified; lane M, DNA molecular size marker (100-bp ladder). No bands were present in the negative control (data not shown). A schematic representation of the fragments amplified is shown on the lefthand side of the figure.