Figure 2.

PLOD1 levels are significantly higher in CD73^– AMSCs versus CD73⁺ AMSCs. (A,B) We examined differential genes between CD73^– AMSCs and CD73⁺ AMSCs. Results are shown in volcano maps in A and B. We found that PLOD1 levels are significantly higher in CD73^– AMSCs versus CD73⁺ AMSCs; (C) we separated CD73^– AMSCs versus CD73⁺ AMSCs by flow cytometry, shown by a representative flow chart; (D) isolated CD73^– AMSCs and CD73⁺ AMSCs in culture; (E) quantitative reverse transcription polymerase chain reaction for CD73 and PLOD1 on CD73^– AMSCs versus CD73⁺ AMSCs. *, P<0.05. n=5. Scale bars are 100 µm. PLOD1, procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1; AMSCs, adipose-derived mesenchymal stem cells; FSC, forward scatter.