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. 2022 Jan;10(2):37. doi: 10.21037/atm-21-6101

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2022 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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MiR-26a directly targets PTEN. (A) Illustration of the binding sites of PTEN and miR-26a. (B) The roles of miR-26a in the luciferase activities of pmirGLO-PTEN-WT and pmirGLO-PTEN-MUT were quantified by luciferase reporter assay. (C) The expression of PTEN in miR-26a overexpressing or depleting cells by qRT-PCR analysis. (D,E) The pictures and statistical diagram of the western blot was shown. Values are the mean ± SD of three independent experiments (n=3). ***P<0.001, ****P<0.0001 versus control. PTEN, phosphatase and tensin homolog; qRT-PCR, quantitative real-time polymerase chain reaction; SD, standard deviation; NC, negative control.