Figure 5.

Altered invasiveness of pancreatic cancer (PaCa) cells after C-X-C motif chemokine ligand 12 (CXCL12) treatment or coculture with fibroblasts (FBs), in the absence or presence of a C-X-C chemokine receptor type 4 (CXCR4) antagonist (AMD070). The invasiveness of normal and radiation-resistant PaCa cell lines was assessed using the double chamber method using a Matrigel invasion assay system. PaCa cells (1×10⁵) were seeded in Matrigel-precoated Transwell chambers and allowed to migrate for 24 h. The effect of adding CXCL12 (100 ng/ml) to the culture medium or coculture with FBs, in the absence of presence of AMD070 (1 µM) treatment, on PaCa cell invasion was also assessed. (A and B) The cells that invaded through the membrane to the bottom of the upper chamber after fixing and staining. Magnification ×100. (C and D) The number of invading cells in nine random microscopic fields. Comparisons for each group were evaluated using two-way analysis of variance with post-hoc Bonferroni tests. *P<0.05.