Fig. 3. miR-34a overexpression significantly suppresses PC3 cell viability and migration in vitro.
A PC3 cells were transfected with pAAVsc CB PI-miR-34a-Gluc for 48 h, and the CCK8 assay was used to evaluate cell viability by measuring the absorbance at 450 nm. B The wound healing assay was performed to investigate the effect of miR-34a on cell migration, and the wound width was recorded at 0 and 24 h using a microscope. C The wound width was statistically analyzed by ImageJ software. *p < 0.05; ****p < 0.0001.