Skip to main content
. 2022 Feb 8;17(9):2007–2013. doi: 10.4103/1673-5374.335163

Figure 2.

Figure 2

Urolithin A attenuates neuronal apoptosis 72 hours following traumatic brain injury.

(A) TUNEL assay (red) and costaining of NeuN (green, Alexa Fluor 488) in injured cortex. TUNEL+/NeuN+ cells are apoptotic neurons. UA administration reduced the number of apoptotic neurons in injured cortex following TBI. Scale bars: 25 µm. (B) Count of apoptotic neurons in the cortex for each group (n = 6). (C) Representative western blots of cleaved caspase-3, caspase-3, and bcl-2. (D) Quantification of relative protein expression (n = 5). Data are represented as mean ± SD. **P < 0.01, ***P < 0.001 (one-way analysis of variance followed by Bonferroni's post hoc test). Cl-caspase3: Cleaved caspase3; n.s.: no statistical significance between indicated groups; NeuN: neuronal nuclei; TBI: traumatic brain injury; TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling; UA: urolithin A; Ve: vehicle.