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. 2022 Feb 16;22:85. doi: 10.1186/s12935-022-02512-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — CD44 and the transcription factor SP-1 is involved in the inhibitory effect of CBS/H₂S axis on CRC cells. A CD44 expression level detected by flow cytometry and quantified through MFI in HT-29 cell line. (*p < 0.05). B Decreased CD44v protein expression after CBS overexpression in HT-29 cell line as well as xenograft samples. C Decreased CD44v mRNA expression after CBS overexpression in HT-29 cell line as well as xenograft samples (***p < 0.001). D Dose-dependent decrease in CD44v protein expression upon GYY4137 supplement. E Elevated CD44v protein expression in HT-29 KO-CBS cell line and Caco-2 sh-CBS cell line. F ChIP assay and following RT-qPCR results showing different level of SP-1 enrichment within the CD44 promoter region in HT-29 control and CBS-overexpressing cells (**p < 0.01). Fold enrichment relative to IgG. G Immunofluorescence assay of SP-1 and DAPI in HT-29 control and CBS-overexpressing cells, depicting SP-1 intracellular distribution. Scale bar, 20 μm. H Analysis of colocalization of SP-1 and DAPI using Pearson's correlation coefficient (**p < 0.01)