Figure 6. Deferoxamine preconditioning increases the expression levels of pro-angiogenic factors. Total RNA was obtained from MSCs exposed to 150 μM DFX, 400 μMDFX, or the vehicle for 48 h and subjected to quantitative reverse transcriptase ± PCR analysis. White bars represent non-preconditioned MSCs, gray bars represent MSCs preconditioned with 150 μM DFX, and black bars represent MSCs preconditioned with 400μMDFX. Data are shown as mean ± SEM. n = 4 per experimental group (biological repeats). Experiments were repeated 3 times at the technical level. *P<0.05. MSCs: Mesenchymal stem cells; DFX: Deferoxamine; PCR: Polymerase chain reaction; VEGFa: Vascular endothelial growth factor-a; ANG-1: Angiopoietin-1; bFGF: Basic fibroblast growth factor; PDGF: Platelet-derived growth factor; SEM: Standard error of the mean.