Fig. 2.

Lack of effect of catecholamine β-adrenoceptor ligands on inflammasome-induced IL-1β secretion from THP-1 cells and primary human microglia. a l-DOPA is catabolized sequentially to DA, norepinephrine, and epinephrine. Image “Biosynthesis of catecholamines” is licensed under CC BY 2.0. b Distribution patterns of DA, norepinephrine, and epinephrine in human brain. Image created in BioRender. c–e Canonical IL-1β production by THP-1 cells is inhibited with 1 mM DA but not by isoprenaline, epinephrine, or norepinephrine at 10 µM (c), 25 µM (d), or 50 µM (e). f Canonical IL-1β production by primary human microglia is also inhibited with 1 mM DA but not by isoprenaline, epinephrine, or norepinephrine at 10 µM. Representative experiments