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. Author manuscript; available in PMC: 2022 Mar 17.
Published in final edited form as: J Am Chem Soc. 2021 Mar 3;143(10):3729–3733. doi: 10.1021/jacs.1c00581

Figure 1.

Figure 1.

16O-18O rR difference spectra of the 17OH-PREG bound CYP17A1 oxy complex without (A)17 and with (B) Mn-Cytb5; the spectrum in trace A was taken from raw data reported in ref 15. The [16O2-18O2] difference trace was generated by subtracting the two absolute spectral traces, where all (many) nonshifted features (i.e., those not associated with the Fe-O-O fragment) effectively cancel, with only the targeted υ(Fe-O) and υ(O-O) modes being observed.