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. 2022 Feb 16;23:74. doi: 10.1186/s12859-022-04593-2

Fig. 4.

Fig. 4

Overview of a CRISPR experiment using CROPSR Timeline and steps of a typical CRISPR/Cas9 knockout experiment in a crop plant genome, utilizing CROPSR. Steps contained in gray blocks represent steps that only need to be done once per genome, at the first utilization of CROPSR (database generation). Consecutive uses on the same genome require only a database search, as shown