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. 2022 Mar;28(3):320–339. doi: 10.1261/rna.078964.121

FIGURE 3.

FIGURE 3.

The yef3-fs1009 mutation suppresses frameshifting at CGA codon repeats when the frame quality control system is compromised by defects in MBF1 or RPS3. (A) Schematic of RFP and GLN4(1–99)-(CGA)4 + 1-GFP reporter used in these analyses. (B–E) The yef3-fs1009 mutation suppresses frameshifting caused by the mbf1-R89K mutation (B), by deletion of MBF1 (C), by the RPS3-K108N mutation (D) or by the RPS3-S104Y mutation (E). In each case, the yef3-fs1009 mutation results in significantly reduced levels of both frameshifted protein and protein per mRNA and in two cases results in small but significant reductions in mRNA. (**) P < 0.01 ≥ 0.001. (***) P < 0.001.