Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar;28(3):371–389. doi: 10.1261/rna.079025.121

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Black and Johnson; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

FIGURE 4. — Bud23 coexists on 40S precursors with the Rps0-cluster proteins. Bud23 and Rio2 associate with pre-40S particles containing the Rps0-cluster proteins as shown by the coimmunoprecipitation of Rps2–FLAG, Rps0, or Rps21-HA with the indicated GFP-tagged baits. (A) Rps2–FLAG coimmunoprecipitated with Bud23–GFP (AJY4722) and Rio2-GFP (AJY4723) but not the Processome factor Utp9-GFP (AJY4721). Asterisks (*) denote the GFP-tagged baits. (B) Rps0 and Rps21A-HA coimmunoprecipitated with Bud23–GFP (AJY4767) and Rio2-GFP (AJY4768) but not Utp9-GFP (AJY4766) nor were they present in a negative control that used cells with no bait (No Tag; AJY4732). The Processome/pre-40S factor Pno1 is shown in A, and the Processome factor Imp4 is shown in B as positive controls for the copurification of preribosomal particles. Rpl8 is shown in B as a specificity control for contamination of mature ribosomes.