Table 1.
Gene | Primer sequence (5′-3′) |
---|---|
OIP5-AS1 | F: 5′-TGCTGTGATGCTGGGAACTT-3′ |
R: 5′-TGGGCTGATTGACCAATCTCA-3’ | |
miR-92a | F: 5′-TATTGCACTTGTCCCGGCCTGT-3’ |
R: mRQ 3’ Primer | |
ITGA6 | F: 5′-GGCGGTGTTATGTCCTGAGTC-3’ |
R: 5′-AATCGCCCATCACAAAAGCTC-3’ | |
E-cadherin | F: 5′-CAGCATCACTGGCCAAGGAGCTGA-3’ |
R: 5′-GACCACACTGATGACTCCTGTGTTCC-3’ | |
Vimentin | F: 5′-CCGACACTCCT ACAAGATTTAGA-3’ |
R: 5′-CAAAGATTTATTGAAGCAGAACC-3’ | |
U6 | F: 5′-GCTTCGGCAGCACATATACTAAAAT-3’ |
R: 5′-CGCTTCACGAATTTGCGTGTCAT-3’ | |
GAPDH | F: 5′-CGGAGTCAACGGATTTGGTCGTAT- 3’ |
R: 5′-AGCCTTCTCCATGGTGGTGAAGAC-3’ |
F forward, R reverse, OIP5-AS1, opa-interacting protein 5 antisense transcript 1, miR-92a, microRNA-92a, ITGA6, integrin alpha 6, GAPDH, glyceraldehyde phosphate dehydrogenase, mRQ 3’ Primer were supplied with Mir-X™ miRNA First-Strand Synthesis and SYBR qRT-PCR kit (Takara Bio Inc., Japan)