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(A) Fluorescence microscopy images of endogenous sphingosine in HeLa cells treated with probe 1 and control probe 2 for 16 h. (B) Quantified fluorescence turn-on of 1 and 2 (final fluorescence [F]/ initial fluorescence [Fi]) within large populations of cells over 24 h. Values are reported as means ± SD. Significance was determined using an unpaired t test: ****P < 0.0001.
