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. 2022 Feb 16;10(1):e01328-21. doi: 10.1128/spectrum.01328-21

FIG 2.

FIG 2

Cytopathic effects caused by long-term HBV infection. (A) 5C-PHHs were infected with HBV (MOI of 200), and samples were collected at 28 dpi and proteomics analysis applied. DAVID analysis of upregulated (left) and downregulated (right) proteins (>1.5-fold change and P < 0.05) against a background of all proteins quantified in the proteomics. Components of all clusters are shown in Table S2 in the supplemental material (B) Integrity toxic analysis of all dysregulated proteins by IPA analysis. (C) 5C-PHHs were infected with HBV at an MOI of 200 for 28 days. Representative TEM photographs showing abnormal mitochondria ultrastructure after long-term HBV infection in hepatocytes. (D) Immunofluorescent analysis of Hoechst 33342 (blue), MitoTracker Green (green), and MitoSOX (red) stain in mock- and HBV-infected 5C-PHH cells. (E) Confocal photographs of Hoechst 33342 (blue) and JC-1 (monomers, green; aggregates, red) stain in mock- and HBV-infected 5C-PHH cells, as indicated.