Comparison of the specificity and sensitivity of RT-qPCR involving the in-house–designed NP gene primer set (iNP) among sputum, nasopharyngeal, saliva, and oropharyngeal samples. (a) Determination and comparison of the specificity and sensitivity of iNP RT-qPCR among sputum, nasopharyngeal, saliva, and oropharyngeal samples at a Ct cutoff of 35 (Ct-35). (b) Determination and comparison of the specificity and sensitivity of iNP RT-qPCR among sputum, nasopharyngeal, saliva, and oropharyngeal samples at a Ct cutoff of 40 (Ct-40).