TABLE 1.
RT-qPCR results on other respiratory viruses and bacteriaa
| Type | Virus/bacteria strain name | RT-qPCR results of different target genes and primer sets (cycle threshold) |
||||||
|---|---|---|---|---|---|---|---|---|
| NP-gene (iNP) | E-gene (Kogene kit) | RdRp-gene (Kogene kit) | WHO RdRp primers | CDC N1 primers | CDC N2 primers | CDC N3 primers | ||
| Virus | Avian infectious bronchitis virus, strain Massachusetts | ND | ND | ND | ND | 35.84 | ND | ND |
| Virus | Human Coronavirus NL63 | ND | ND | ND | ND | 38.47 | ND | ND |
| Virus | Canine coronavirus Strain UCD1 | ND | ND | ND | ND | 37.04 | ND | ND |
| Virus | MERS-CoV | ND | ND | ND | UD | ND | ND | ND |
| Virus | SARS-CoV Purified, in PBS 1 × 10^8 pfu/mL (eq), Urbani strain | ND | 24.23 | ND | 31.76 | 38.08 | 30.10 | 25.36 |
| Virus | Human respiratory syncytial virus, Strain A2000/3-4 | ND | ND | ND | ND | 36.47 | 38.95 | ND |
| Virus | Influenza A/Texas/36/91, H1N1 | ND | ND | ND | 35.51 | 37.55 | 38.57 | ND |
| Virus | Influenza B/Florida/4/2006 | ND | ND | ND | ND | ND | 37.81 | ND |
| Virus | Influenza C virus C/Taylor/1233/1947 | ND | ND | ND | ND | 39.17 | 39.03 | ND |
| Virus | Measles virus Edmonston | ND | ND | ND | ND | ND | 39.02 | ND |
| Virus | Rhinovirus | ND | ND | ND | ND | ND | ND | ND |
| Virus | Human astrovirus (HAstV) type 1 | ND | ND | ND | ND | ND | ND | ND |
| Virus | Human astrovirus (HAstV) type 2 | ND | ND | ND | ND | 36.34 | ND | ND |
| Bacteria | Klebsiella pneumoniae Isolate 1 | ND | ND | ND | ND | 38.70 | ND | ND |
| Bacteria | Klebsiella oxytoca MIT 10-5244 | ND | ND | ND | ND | 37.16 | ND | ND |
| Bacteria | Leptospira interrogans HAI0156 (Serovar Copenhageni) | ND | ND | ND | ND | 37.13 | ND | ND |
| Bacteria | Mycobacterium abscessus #103 | ND | ND | ND | ND | 37.37 | ND | ND |
| Bacteria | Mycobacterium avium 2285 Smooth | ND | ND | ND | ND | 37.10 | ND | ND |
| Bacteria | Mycobacterium intracellulare 1956 | ND | ND | ND | ND | 37.30 | ND | ND |
| Bacteria | Staphylococcus aureus Strain AIS 1000505 AKA VRS10 | ND | ND | ND | ND | 37.73 | ND | ND |
| Bacteria | Staphylococcus; aureus MRSA; M0200 | ND | ND | ND | ND | 37.02 | ND | ND |
| Bacteria | Streptococcus pneumoniae Strain TCH8431 | ND | ND | ND | ND | ND | ND | ND |
| Bacteria | Pseudomonas aeruginosa ATCC 27853 | ND | ND | ND | ND | ND | ND | ND |
a
ND, not detectable; cycle threshold values are presented as obtained here. Cycle threshold >35 is considered a negative result for the Kogene Kit; iNP, the in-house–designed primer set targeting the NP gene.