TABLE 2.
Primers used for the analysis of gene expression in this work
| Primer | Sequence (5′–3′)a | PCR product size (bp) | Reference or source |
|---|---|---|---|
| RpsL-1 | GCTGCAAAACTGCCCGCAACG | 250 | 66 |
| RpsL-2 | ACCCGAGGTGTCCAGCGAACC | ||
| AC-RNA-F | GGGCTGGCCTCGAAAGAGGAC | 246 | 67 |
| AC-RNA-R | GCACCGAGTCGGGGAACTGCA | ||
| VIM-1-RNA-F | AGATTGCCGATGGTGTTTGGT | 265 | This work |
| VIM-1-RNA-R | GATGCGTACGTTGCCACCC | ||
| GES-1-RNA-F | AGCGGTTTCTAGCATCGGGA | 260 | This work |
| GES-1-RNA-R | CATAGAGGACTTTAGCCACAG | ||
| OXA-2-RNA-Fb | TTTTCGATGGGACGGCGTTAA | 256 | This work |
| OXA-2-RNA-R | ATAGAGCTTCCTGAGAAATGCA |
a
Sequences were obtained from the published PAO1 genome or from sequences of blaVIM-1, blaGES-1, or blaOXA-2 and derivatives deposited in GenBank.
b
Since the hybridization site for these primers was conserved for OXA-2 and the three OXA-2 derivatives studied in this work, this same pair of primers was used to quantify the expression of all of them.