FIG 5.

Each graph (A to D) represents viral titers of supernatant collected from US/MO/14-18947 grown in a neuroblastoma cell line (SH-SY5Y) and collected at four consecutive time points at 3-day intervals (day 3, 6, 9, and 12). The y axis represents the TCID₅₀ at each density divided by the total TCID₅₀ for the whole sample. Error bars represent SEM across 3 biological replicates. Average “total TCID₅₀” viral titers for each time point are shown in the box at the top of each chart. To the right of each graph is a representative image of virus-infected SH-SY5Y cells for which viability was assessed using an MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. Metabolically active cells turn dark blue in the presence of MTT.