Figure 3.

C19-RBCs Induce Upregulation of Vascular Arginase 1 and 4-HNE

Representative immune-histochemical images for arginase 1 (A) and 4-hydroxynonenal (4-HNE) (D) in aortic rings incubated with either H-RBCs or C19-RBCs for 18 hours. Immunoglobulin G controls are presented in inserts for each experimental condition. Quantitative analyses of arginase 1 and 4-HNE positivity of the total area (B and E) (n = 5-6) and the endothelial lining (C and F) (n = 5-6). L = luminal side of the vessel, black arrows = endothelial cells, and red arrows = smooth muscle cells. ∗P < 0.05, ∗∗P < 0.01 with Mann-Whitney test. Values are expressed as median (Q1-Q3). Abbreviations as in Figure 2.