TABLE 2.
Comparison of culture and RT-qPCR assay of spiked blinded surveillance samplesa
| RT-qPCR | No. of surveillance samples spiked with indicated culture result |
Accuracy (%) | Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) | |||
|---|---|---|---|---|---|---|---|---|---|
| Live C. auris (positive) | Live other Candida spp. (positive) | Dead C. auris (negative) | No organism (negative) | ||||||
| No. positive | 15 | 0 | 0 | 0 | 93 | 88 | 100 | 100 | 87 |
| No. negative | 2 | 6b | 4b | 3b | |||||
a
PPV, positive predictive value; NPV, negative predictive value.
b
Samples spiked with dead C. auris or other organisms and those spiked with no organisms are considered true-negative samples in this analysis; thus, the number of true-negative samples is 13 in the subsequent calculations.