TABLE 2.

Sample carryover test results of HBsAg NEXT^a

Run	Mean S/CO of:			S/CO difference between unprotected and protected negative samples	P valueb (unprotected versus protected)
	Protected negative	High positive sample	Unprotected negative
1	0.20	946.68	0.21	0.01	0.21
2	0.19	954.15	0.18	−0.01	0.54

^aData were generated from two runs, each with 5 replicates of each sample. Protected negative and unprotected negative samples were run preceding and following the high-positive sample, respectively. S/CO, signal over cutoff.

^bP value was determined by t test. A P value of <0.05 was considered statistically significant.