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. 2022 Feb 16;8(7):eabk3234. doi: 10.1126/sciadv.abk3234

Fig. 1. Melanoma cells trigger rapid CTLs lytic granules exposure.

Fig. 1.

(A) D10 cells were either left unpulsed or pulsed with different concentrations of antigenic peptide as indicated and conjugated with cognate CTLs for 2, 5, or 15 min. Following conjugation, CD107a exposure on the CTL surface was quantified using flow cytometry. Data shown indicate percentages of CD107a+ CTLs. (B to D) CTL and melanoma cells were conjugated in the presence of FM1-43 (10 μg/ml) in the medium. (B) Representative flow cytometry plots of FM1-43 fluorescence intensity in CTLs. (C) Time kinetics of FM1-43 fluorescence intensity in CTLs conjugated with D10, EB81, M17, and LB2259 melanoma cells are shown. (D) D10 cells were either left unpulsed or pulsed with different concentrations of antigenic peptide as indicated and conjugated with cognate CTL for 2, 5, or 15 min in the presence of FM1-43 (10 μg/ml) in the cell culture medium. Numbers indicate percentages of FM1-43+ CTLs based on the condition without peptide. Results shown are from one experiment representative of two (A and D) or five (B and C) independent experiments. Data are expressed as means ± SEM. Two-way analysis of variance (ANOVA) using GraphPad Prism was used to assess statistical significance of differences. ****P < 0.0001.