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. 2022 Feb 16;8(7):eabm1418. doi: 10.1126/sciadv.abm1418

Fig. 3. Mechanism exploration for iLAND-mediated siRNA transfection.

Fig. 3.

(A) Subcellular localization of Cy5-siRNA@iLAND in HepG2 cells receiving various treatments. (B) Relative MFI of Cy5-siRNA recorded in (A). (C) Cellular uptake of Cy5-siRNA@iLAND in HepG2 cells as recorded by fluorescence-activated cell sorting (FACS). (D) Quantitative analysis of (C). (E) Confocal observation of the internalization of Cy5-siRNA@iLAND in HeLa cells. ApoE and calreticulin were used to determine whether they bound with iLAND and facilitated the cellular entry of Cy5-siRNA@iLAND. DMEM, Dulbecco’s modified Eagle’s medium; FBSDMEM, DMEM containing 10% fetal bovine serum. (F) Quantitative analysis of the fluorescence intensity of siRNA as recorded in (E). (G and H) Cellular uptake of Cy5-siRNA@iLAND in HeLa cells as recorded by FACS. siRNA was transfected at a final concentration of 50 nM. Scale bars, 20 μm.