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. 2022 Feb 4;18(2):e1009860. doi: 10.1371/journal.pcbi.1009860

Fig 1. Scaffolding the Athal-Illumina assembly with LINKS, SMIS, LRScaf, and SAMBA.

Fig 1

We compared performance as a function of long-read (PacBio) coverage, shown on the x-axis. Panels (a) and (b) show the NGA50 size for contigs and scaffolds after scaffolding with each tool. SAMBA outputs contigs, and thus its scaffold and contig results are the same. Panels (c) and (d) show the number of mis-assemblies introduced by the scaffolding process. The LINKS scaffolder required more than 512GB of RAM for PacBio coverage >20x, and thus we did not run it with higher coverage. SMIS does not change contigs, so we did not include its statistics on panels (a) and (c).