KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal anti-Myc Y69 | Abcam | Cat# ab32072 |
| Mouse monoclonal Pan-Ras | Sigma-Aldrich | Cat# OP40 |
| Mouse monoclonal anti-GAPDH | Ambion | Cat# AM4300 |
| Anti-rabbit HRP | GE Healthcare Life Sciences | Cat# NA934 |
| Anti-mouse HRP | GE Healthcare Life Sciences | Cat# NA931V |
| Chemicals, peptides, and recombinant proteins | ||
| β-estradiol (E2) | Sigma-Aldrich | Cat# E8875 |
| Doxycycline | Sigma-Aldrich | Cat# D3447 |
| Zeocin | InvivoGen | Cat# ant-ZN |
| Dexamethasone | Sigma-Aldrich | Cat# D1756 |
| Agar (for colony formation assay) | Sigma-Aldrich | Cat# A1296 |
| JF646 HaloTag ligand | Grimm et al., 2017 | N/A |
| 4-thiouridine (4sU) | Sigma-Aldrich | Cat# T4509 |
| Iodoacetamide (IAA) | Sigma-Aldrich | Cat# I1149 |
| TRIzol Reagent | Invitrogen | Cat# 15596018 |
| Critical commercial assays | ||
| Virabind Lentivirus Purification Kit | CellBioLabs | Cat# VPK-104 |
| iQ SYBR Green Supermix | Bio-Rad | Cat# 170880 |
| Deposited data | ||
| SLAM-Seq | This manuscript | GEO: GSE189943 |
| Experimental models: Cell lines | ||
| U2OS MS2-PP7 Beta-globin reporter | Coulon et al., 2014 | N/A |
| MCF7-TFF1 MS2 | Rodriguez et al., 2019 | N/A |
| MCF7 TetON MYC-EGFP | Eric Batchelor Lab | N/A |
| NIH3T3 | ATCC | Cat# CRL-1658 |
| NIH3T3 HRAS V12/Pi-MYC | This manuscript | N/A |
| NIH3T3 HRAS V12/Pi-mCherry | This manuscript | N/A |
| HBEC3-KT | ATCC | Cat# CRL-4051 |
| HBEC3-KT with intronic MS2 stem loops (polyclonal population) | Wan et al., 2021 | N/A |
| HBEC3-KT with intronic MS2 stem loops (single cell clones) | Wan et al., 2021 | N/A |
| HBEC3-KT TetON MYC-EGFP | This manuscript | N/A |
| HBEC3-KT MS2 polyclonal / Pi-MYC | This manuscript | N/A |
| HBEC3-KT MS2 polyclonal / Pi-mCherry | This manuscript | N/A |
| HBEC3-KT MS2 single cell clones / Pi-MYC | This manuscript | N/A |
| HBEC3-KT MS2 single cell clones / Pi-mCherry | This manuscript | N/A |
| Oligonucleotides | ||
| qPCR primers | IDT | SeeTable S1 |
| Single-molecule RNA FISH probes | LGC Biosearch Technologies | SeeTable S1 |
| Recombinant DNA | ||
| pTRIEX-mCherry-LOV2 | Addgene | Cat# 81041 |
| pd4-MYC-EGFP | Nie et al., 2012 | N/A |
| pBABE-neo-HRAS-V12 | Scaffidi and Misteli, 2011 | N/A |
| pTRIPZ TetON MYC-EGFP | David Levens Lab | N/A |
| pLenti-Pi-Myc | This manuscript | N/A |
| pLenti-Pi-mCherry | This manuscript | N/A |
| pHaloTag-GR | Morisaki et al., 2014 | N/A |
| pHaloTag-H2B | Mazza et al., 2012 | N/A |
| pFC15A-HaloTag-MED1 | Gordon Hager Lab | N/A |
| pFN21A-HaloTag-TBP | Promega | Cat# FHC11099 |
| pFN21A-HaloTag-SUPT5H (SPT5) | Promega | Cat# FHC02125 |
| pFN21A-HaloTag-POLR2A | Promega | Cat# FHC28428 |
| pFC15A-HaloTag-MYC | This manuscript | N/A |
| pFC15A-HaloTag-MYC-S62A | This manuscript | N/A |
| Software and algorithms | ||
| CellProfiler | Carpenter et al., 2006 | https://cellprofiler.org |
| Localize | Larson et al., 2013 | https://github.com/CBIIT/Larson-Lab-CCR-NCI/tree/master |
| FISHAuxiliary | Larson et al., 2013 | https://github.com/CBIIT/Larson-Lab-CCR-NCI/tree/master |
| KNIME-based image analysis pipeline | Wan et al., 2021 | https://github.com/CBIIT/Larson-Lab-CCR-NCI/tree/master |
| MATLAB | The MathWorks, Inc. | https://www.mathworks.com |
| MatlabTrack_v6 | Ball et al., 2016 | https://sourceforge.net/projects/single-molecule-tracking |