Fig. 3 |. HR mammary epithelial progenitors show a differentiation defect and a basal differentiation bias.

a–d, Immunofluorescent images of cKit-enriched cells stained for KRT19 (green) and KRT14 (red) that were fixed at 2 d of culture from an AR younger woman (a), an AR older woman (b), an HR 35-year-old woman harboring a BRCA1 mutation (BRCA1^mut) (c) and an HR 44 year-old woman harboring a BRCA2^mut (d). Scale bars, 50 μm. e–j, Density contour plots of KRT14 and KRT19 mean florescent intensity signals in cKit-enriched cells that were fixed after 2 d of culture from AR younger women (e), AR older women (f) and HR women harboring germline mutations of BRCA1 (g), BRCA2 (h), PALB2 (i) and PALB2(VUS) + APC(VUS) (j). Experiments in a–d were repeated three times independently with similar results. The number of individuals with cKit⁺ were derived from representing each group as follows: a, three; b, two; c, eight; d, six.