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. Author manuscript; available in PMC: 2022 Jul 13.
Published in final edited form as: Nat Cell Biol. 2022 Jan 13;24(1):51–61. doi: 10.1038/s41556-021-00812-9

Extended Data Fig. 9. SHLD1Δ suppresses overhang length at TPP1-deficient telomeres. Related to Fig. 6.

Extended Data Fig. 9

a, Immunoblot showing expression of 53BP1 pathway components and SHLD1 construct expression in Tpp1F/F Shld1+/+ or Shld1−/− clones. b, Immunoblot showing bulk CRISPR KO of BRCA1 and SHLD1 construct expression in cells of the indicated genotype with SHLD1 construct expression. c, Quantitative analysis of telomeric ss overhang intensity in cells as in b using in-gel hybridization to detect the 3’ overhang followed by rehybridization to the denatured DNA in the same gel, allowing the ratio of ss to total TTAGGG signal to be determined. d, Immunoprecipitation of SHLD1 in Tpp1F/F MEFs with the indicated treatments and STN1 immunoblot. Experiments in a-d have been performed once.