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. 2022 Feb 17;298(4):101739. doi: 10.1016/j.jbc.2022.101739

Figure 1.

Figure 1

Structure and names of fluorescent substrates used in this study.A, fluorogenic VKLQ–AMC substrate, with AMC fluorophore shown in blue. B, nsp4–5-EDANS FRET substrate uses an EDANS fluorophore shown in blue, and a 4-(4-dimethylaminophenylazo)benzoic acid (DABCYL) quencher shown in gray. C, nsp4–5-MCA FRET substrate uses an MCA fluorophore shown in purple with a 2,4-dinitrophenyl (DNP) quencher shown in gray. D, nsp4–5-FAM FRET substrate uses a FAM fluorophore shown in green with a DABCYL quencher shown in gray. The red dashed line indicates the cleavage site on the substrates. AMC, 7-amino-4-methylcoumarin; EDANS, 5-(2-aminoethylamino)-1-naphthalenesulfonic acid; FAM, 5-carboxyfluorescein; MCA, 7-methoxycoumarin-4-acetic acid.