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Michaelis–Menten plot for the VKLQ–AMC substrate. Values of 172 ± 28 μM, 0.842 ± 65 nM s−1; and 0.00421 ± 53 s−1 are measured for the Km, Vmax, and kcat, respectively. kcat/Km was found to be 24.5 ± 5.0 M−1 s−1. Each data point is the mean with error bars showing ±1 standard deviation, n = 3. AMC, 7-amino-4-methylcoumarin.
