Table 1.
Substrate | Sequencea | kcat/Km (M−1 s−1) |
---|---|---|
AKLQ–AMC | Ac-VKLQ ↓ {AMC} | 18.5 ± 1.0 |
24.5 ± 5.0b | ||
nsp4–5-MCA | {MCA}-AVLQ ↓ SGFR{K(DNP)}K-NH2 | 14,190 ± 420 |
nsp4–5-EDANS | {DABCYL}-KTSAVLQ ↓ SGFRKM{E(EDANS)}-NH2 | 1960 ± 190 |
nsp4–5-FAM | {DABCYL}-KTSAVLQ ↓ SGFR{K(FAM)}K-NH2 | 2448 ± 85 |
nsp5–6-FAM | {DABCYL}-KSGVTFQ ↓ SAVK{K(FAM)}K-NH2 | 77.5 ± 4.2 |
nsp6–7-FAM | {DABCYL}-KKVATVQ ↓ SKMS{K(FAM)}K-NH2 | 68 ± 10 |
nsp8–9-FAM | {DABCYL}-KSAVKLQ ↓ NNEL{K(FAM)}K-NH2 | 6.01 ± 0.61 |
nsp10–12-FAM | {DABCYL}-KREPMLQ ↓ SADA{K(FAM)}K-NH2 | 4.74 ± 0.48 |
nsp14–15-FAM | {DABCYL}-KTFTRLQ ↓ SLEN{K(FAM)}K-NH2 | 38.0 ± 5.2 |
Text in brackets denotes fluorophore or quencher, ↓ denotes cleavage site.
Kinetic parameters determined from Michaelis–Menten plot. Each value reported as the mean ± 1 standard deviation, n = 3.