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. 2022 Feb 17;298(4):101739. doi: 10.1016/j.jbc.2022.101739

Table 1.

Steady-state kinetic parameters for SARS-CoV-2 Mpro fluorescent substrates

Substrate Sequencea kcat/Km (M−1 s−1)
AKLQ–AMC Ac-VKLQ ↓ {AMC} 18.5 ± 1.0
24.5 ± 5.0b
nsp4–5-MCA {MCA}-AVLQ ↓ SGFR{K(DNP)}K-NH2 14,190 ± 420
nsp4–5-EDANS {DABCYL}-KTSAVLQ ↓ SGFRKM{E(EDANS)}-NH2 1960 ± 190
nsp4–5-FAM {DABCYL}-KTSAVLQ ↓ SGFR{K(FAM)}K-NH2 2448 ± 85
nsp5–6-FAM {DABCYL}-KSGVTFQ ↓ SAVK{K(FAM)}K-NH2 77.5 ± 4.2
nsp6–7-FAM {DABCYL}-KKVATVQ ↓ SKMS{K(FAM)}K-NH2 68 ± 10
nsp8–9-FAM {DABCYL}-KSAVKLQ ↓ NNEL{K(FAM)}K-NH2 6.01 ± 0.61
nsp10–12-FAM {DABCYL}-KREPMLQ ↓ SADA{K(FAM)}K-NH2 4.74 ± 0.48
nsp14–15-FAM {DABCYL}-KTFTRLQ ↓ SLEN{K(FAM)}K-NH2 38.0 ± 5.2
a

Text in brackets denotes fluorophore or quencher, ↓ denotes cleavage site.

b

Kinetic parameters determined from Michaelis–Menten plot. Each value reported as the mean ± 1 standard deviation, n = 3.