Figure 7.

CEP increased cell viability and inhibited lipid peroxidation of bEnd.3 at 24 h after RSL3 exposure, and systematic inhibition of AA metabolizing enzymes identified ALOX15 as a target for ferroptosis. (a) RSL3 decreased cell viability detected by CCK-8. ^∗p < 0.05 vs. control, n = 6 per group. (b) The CCK-8 results depicted that CEP saves RSL3-induced cell death in a concentration-dependent manner. ^∗p < 0.05 vs. control, ^#p < 0.05 vs. DMSO group, n = 6 per group. (c) Representative morphological images of bEnd.3 cells exposed to RSL3 with or without CEP. Scale bar = 20 μm. (d, e) Representative picture of BODIPY 581/591 C11 staining and quantitative fluorescence ratio analysis of green (representing lipid peroxidation)/red (conventional coloring). (f) The table clarified that inhibitors of 15-lipoxygenase and 5-lipoxygenase, but not other types of oxygenase which catalyze AA, suppressed RSL3-induced ferroptosis in bEnd.3 cells.