Figure 8.

Figure showing connection between microdissection and epigenetic analysis as published by our group [42]. (a) Schematic diagram showing experimental design of miR-200b promoter methylation analysis in endothelial elements collected from human chronic wounds. (b) Representative figure shows the selection of CD31⁺ tissue elements (red) and their collection before and after the laser capture microdissection (LCM). Scale bar, 150 μm. (c, d) Methylation profile and quantitation of methylated CpG islands present in miR-200b promoter in diabetic wounds compared to normoglycemic wounds. Reproduced with permission from the American Society of Gene and Cell Therapy. The following original report was credited: Singh et al. [42].