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Density of spirochetes in tick midguts determined by q-PCR. Spirochete numbers were determined for pools containing 15 midguts dissected from 15 individual ticks. The total in a pool was divided by 15. Three trials were conducted for each time point; bars represent the averages for the three trials, and lines represent the standard deviations; an asterisk indicates a P of <0.05 as determined by Student's t test in comparison with the values observed at 72 h.
