Table 1.
Sequence of primers used in RT-qPCR.
| Species | Gene | Primer sequence (5′-3′) |
|---|---|---|
| Human |
ACTB |
F:TGTTACAGGAAGTCCCTTGCC |
| R:ATGCTATCACCTCCCCTGTGTG | ||
|
EZH2 |
F:GTACACGGGGATAGAGAATGTGG |
|
| R:GGTGGGCGGCTTTCTTTATCA | ||
|
FASN |
F:ACAGCGGGGAATGGGTACT |
|
| R:GACTGGTACAACGAGCGGAT | ||
|
ACLY |
F:GCTCAGCCAGAACTTGGTAGTC |
|
| R:CTTGCCAACTGTGGCTTCCTG | ||
|
ACACA |
F:CCTGAAGACCTTAAAGCCAATGC |
|
| R:AGCCCACACTGCTTGTACTG | ||
|
ACACB |
F:GGGTCATCGAGAAGGTGCTTAT |
|
| R:GATGTACTCTGCGTTGGCCTTA | ||
|
SCD |
F:TCCAGAGGAGGTACTACAAACCT |
|
| R:CCGGGGGCTAATGTTCTTGT | ||
|
ABCA1 |
F:CTCCACAAGGTATTTTTGCAAGGC |
|
| R:AGTTTAGTGTTCTCAGGATTGGCT | ||
|
ACSL6 |
F:AAGATTCAGGCCAGTCTTGGTG |
|
| R:TAACCTTCATAAACCTGGCACCC | ||
|
ELOVL2 |
F:ATGTTTGGACCGCGAGATTCT |
|
| R:CCCAGCCATATTGAGAGCAGATA | ||
| Mouse |
Actb |
F:AGGGTGTGATGGTGGGAATG |
| R:CCAGTTGGTAACAATGCCATGT | ||
|
Ezh2 |
F:GCATGGTGACAGAGAATGTGG |
|
| R:ATTTCCGAGGTGGGCAAGTT | ||
|
Acsl6 |
F:AAGAAGTGGAGGACGGTGGT |
|
| R:GGGTCCATTCCCTGAGATGCTA | ||
|
Abca1 |
F:ACATGAGTGCCACTTTCCGAAT |
|
| R:GCGAGACACGATGGACTTGTT | ||
|
Slc27a1 |
F:GGCATGGATGATCGGCTGTTT |
|
| R:TGATGTTCCCTGCAGAGTGGTA | ||
|
Fasn |
F:GACCTCAGGCTGCAGTGAAT |
|
| R:TGGTTCCCGGATCACCTTCT | ||
|
Scd1 |
F:TCCCTCCGGAAATGAACGAG |
|
| R:GTAGAAAATCCCGAAGAGGCAGG | ||
| Elovl2 | F:CCTGCTCTCGATATGGCTGG |
|
| R:AAGAAGTGTGATTGCGAGGTTAT | ||
Data were analyzed by ΔΔCt method and gene expression was normalized to ACTB or Actb.